| 1. | Construction and identification of dna vaccine containing chimeric gene gag - gp120 of hiv
120嵌合基因核酸疫苗的构建与鉴定 |
| 2. | Finally , the chimeric gene was inserted into binary ti vector plbj21 , and cloned into agrobacterium tumefaciensgv3101 by electroporation
为了提高can小肽表达强度，本实验构建了can小肽二连体。 |
| 3. | To investigate the molecular basis of the stresses - induced gene regulation , the acbadh promoter - / ? - glucuronidase chimeric gene constructs containing six deletions were introduce into tobacco by agrobacterium - mediated transformation
对启动子进行6禾缺夫构建，瞬时转化烟草叶片表明， 6种构建都有活性。 |
| 4. | In transgenic tobacco plants , the analysis of transient expression by monitoring 3 - glucuronidase activity revealed that a chimeric gene construct containing a 1 . 2 kb pdf1 . 2 promoter fused to a gus reporter gene was induced by meja
( 1 )从拟南芥基因组中扩增出长度约为1200bp的pdf1 . 2启动子，与gus构建的融合基因在烟草中的表达受meja诱导。 |
| 5. | To target this mitochondrial enzyme into chloroplast , the cdna sequence of mnsod was fused to a chloroplast transit peptide from a pea rubisco small subunit gene , whereas expression of the chimeric gene was driven by the camv 35s promoter
Pchlsod质粒含有烟草mnsod基因的cdna序列，与豌豆核糖体小亚基叶绿体引物肽( tp )的编码基因序列构成融合基因，由35s启动子调控。 npt基因为选择标记基因， pgv2260为辅助质粒。 |
| 6. | They indicated that ecbp21 may have some relative with the response to stress treatments . further more , we constructed the binary vector containing ecbp21 - gfp chimeric gene and got transgenic plants by using the agrobacterium vacuum infiltration method
4 .初步开展了转基因研究:为了进一步确定ecbp21在植物生长发育过程中的作用，构建了ecbp21植物表达二元载体，通过真空渗透法转化了拟南芥并获得了转基因植株。 |
| 7. | To get in vivo evidences that apoplast calmodulin con 1d regulate plant growth and development process , a chimeric secretion form of calmodulin binding peptide , which contains a signal peptide , a calmodulin binding domain and a c - myc epitope was constructed . the chimeric gene was introduced into arabidopsis . it was expected that the overexpression of this chimeric protein could be secreted into cell wall and bound to apoplast calmodulin , which could reduce the apoplast calmoduin concentration to make an apoplast camodulin " antisense " plant . by observing the potential phenotype change of apoplast calmodulin " antisense " plant , the in vivo function of apoplast calmodulin on plant growth and developmental process could be speculated
但这些多是采用生理学手段和药理学方法而得出的体外( invitro )实验结果，为了取得质外体cam在植物生长发育过程中发挥重要作用的invivo实验证据，根据动物中的一些研究方法，本实验设计并构建了带有信号肽、 cam结合肽( can小肽) 、 epitope ( c - myc )融合基因的载体，并将融合基因通过真空渗入法转入拟南芥，预期过表达的融合蛋白将会被分泌到细胞外并与质外体cam相结合，这样就会抑制质外体cam的功能，从而可以构建质外体cam的“反义”植株，通过观察质外体cam “反义植株”的表型改变，就可以推断质外体cam在植物生长发育过程中的功能。 |
| 8. | The chimeric gene was transformed into rice calli by particle bombardment . many resistance calli were obtained , and gus activity was detected . in order to confirm the causality of the mutant phenotype and osdd2 gene , we took an approach of functional knockout ofosdd2 gene , with the method rnai ( rna interference )
为了进一步验证本基因的功能，利用获得的包含wrky保守域的cdna片断构建了可能产生rna干涉( rnainterference ， rnai )的载体，利用农杆菌转化法转化水稻，目前已经获得一批转基因苗。 |
| 9. | The chimeric gene was introduced into arabidopsis through the method of vacuum infiltration which is widely used in arobidopsis tansformation . transgenic plants were screened by means of kanamycin resistance and further identified by genomic pcr and western blotting , through the phenotype observation of transgenic plants , the speculation that apoplast calmodulin may play a certain role in regulating the development and growth of plants was made
最后，融合基因被插入二元载体plbj21 ，通过电击法转入农杆菌gv3101 ，真空渗入法转入了拟南芥，转基因拟南芥通过kan抗性筛选而得到，并进一步进行了基因组pcr鉴定和western杂交的鉴定。 |
| 10. | In transgenic tobacco plants , the transient - expression assay of the chimeric gene ( 4 x gcc - 35s min : : gus ) demonstrated that the 4 x gcc - 35smin promoter could respond to meja treatment and the gcc box is an important element in response to ja signaling . moreover , this experiment results would be meaningful to improve the crops characterization of resistance against various environment stresses or to study the regulation of gene expression in transgenic plants
（ 3 ）以反向的4xgcc重复序列（ placzi 4xgcc （ 》作为placzi4xgcc的突变体， 6半乳糖苦酶活性分析的结果表明，与野生型的相比，突变的gcc元件不能与jerfi 2 3 4相互作用， p半乳糖苦酶实验不能呈现出蓝色反应；证明gccbox与jerf 2 3 4是特异性结合。 |
